ThaumatinV1, Main, Exploration, bibRecord, 000079

Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.

Identifieur interne : 000079 ( Main/Exploration ); précédent : 000078; suivant : 000080

Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.

Auteurs : Rongrong He [République populaire de Chine, États-Unis] ; Jiao Wu [République populaire de Chine] ; Yali Zhang [République populaire de Chine] ; Cecilia B. Agüero [États-Unis] ; Xinlong Li [République populaire de Chine] ; Shaoli Liu [République populaire de Chine] ; Chaoxia Wang [République populaire de Chine] ; M Andrew Walker [États-Unis] ; Jiang Lu [République populaire de Chine]

Source :

Protoplasma [ 1615-6102 ] ; 2017.

RBID : pubmed:27900595

Descripteurs français

KwdFr :
- Activation de la transcription (MeSH), Amélioration génétique (MeSH), Expression des gènes (MeSH), Feuilles de plante (génétique), Feuilles de plante (microbiologie), Hyphae (croissance et développement), Maladies des plantes (microbiologie), Oomycetes (croissance et développement), Protéines végétales (génétique), Protéines végétales (métabolisme), Résistance à la maladie (MeSH), Vitis (génétique), Vitis (microbiologie), Végétaux génétiquement modifiés (génétique).
MESH :
- croissance et développement : Hyphae, Oomycetes.
- génétique : Feuilles de plante, Protéines végétales, Vitis, Végétaux génétiquement modifiés.
- microbiologie : Feuilles de plante, Maladies des plantes, Vitis.
- métabolisme : Protéines végétales.
- Activation de la transcription, Amélioration génétique, Expression des gènes, Résistance à la maladie.

English descriptors

KwdEn :
- Disease Resistance (MeSH), Gene Expression (MeSH), Genetic Enhancement (MeSH), Hyphae (growth & development), Oomycetes (growth & development), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Transcriptional Activation (MeSH), Vitis (genetics), Vitis (microbiology).
MESH :
- chemical , genetics : Plant Proteins.
- genetics : Plant Leaves, Plants, Genetically Modified, Vitis.
- growth & development : Hyphae, Oomycetes.
- chemical , metabolism : Plant Proteins.
- microbiology : Plant Diseases, Plant Leaves, Vitis.
- Disease Resistance, Gene Expression, Genetic Enhancement, Transcriptional Activation.

Abstract

Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "Zuoshan-1," a clonal selection from wild Vitis amurensis Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of V. vinifera 'Thompson Seedless' via Agrobacterium tumefaciens. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with Plasmopara viticola, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to P. viticola in transgenic 'Thompson Seedless' grapevines.

DOI: 10.1007/s00709-016-1047-y
PubMed: 27900595

Affiliations:

République populaire de Chine, États-Unis

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000098
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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (MeSH)</term>
<term>Gene Expression (MeSH)</term>
<term>Genetic Enhancement (MeSH)</term>
<term>Hyphae (growth & development)</term>
<term>Oomycetes (growth & development)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Transcriptional Activation (MeSH)</term>
<term>Vitis (genetics)</term>
<term>Vitis (microbiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Activation de la transcription (MeSH)</term>
<term>Amélioration génétique (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (microbiologie)</term>
<term>Hyphae (croissance et développement)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Oomycetes (croissance et développement)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Résistance à la maladie (MeSH)</term>
<term>Vitis (génétique)</term>
<term>Vitis (microbiologie)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
</keywords>
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<term>Oomycetes</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term>
<term>Plants, Genetically Modified</term>
<term>Vitis</term>
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<term>Oomycetes</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term>
<term>Protéines végétales</term>
<term>Vitis</term>
<term>Végétaux génétiquement modifiés</term>
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</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term>
<term>Maladies des plantes</term>
<term>Vitis</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
<term>Plant Leaves</term>
<term>Vitis</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term>
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<keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term>
<term>Gene Expression</term>
<term>Genetic Enhancement</term>
<term>Transcriptional Activation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Activation de la transcription</term>
<term>Amélioration génétique</term>
<term>Expression des gènes</term>
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<front><div type="abstract" xml:lang="en">Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "Zuoshan-1," a clonal selection from wild Vitis amurensis Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of V. vinifera 'Thompson Seedless' via Agrobacterium tumefaciens. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with Plasmopara viticola, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to P. viticola in transgenic 'Thompson Seedless' grapevines.</div>
</front>
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<DateCompleted><Year>2018</Year>
<Month>04</Month>
<Day>06</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
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<Issue>4</Issue>
<PubDate><Year>2017</Year>
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<Title>Protoplasma</Title>
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<ArticleTitle>Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.</ArticleTitle>
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<Abstract><AbstractText>Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "Zuoshan-1," a clonal selection from wild Vitis amurensis Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of V. vinifera 'Thompson Seedless' via Agrobacterium tumefaciens. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with Plasmopara viticola, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to P. viticola in transgenic 'Thompson Seedless' grapevines.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Rongrong</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Viticulture and Enology, University of California, Davis, Davis, CA, 95616, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Jiao</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Yali</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>The Viticulture and Enology Program, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Agüero</LastName>
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Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.

Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine.

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